Hotspot

trnF-GAA-ndhJ

LSC · 49408-49793 · 386 bp

Back to Acronema

Pi 0.0383

MarkerSeek score 75.70

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 386
variable_sites: 63
pi: 0.0383
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	TGGATAGGATGGCCTTTACG	55.0	50.0	58.0	56.8	518-543 bp	1.0	82.0
trnF-GAA-ndhJ_p2	GGGATAGCTCAGCTGGTAGA	GGATAGGATGGCCTTTACGT	55.0	50.0	58.0	56.8	517-542 bp	1.0	82.0
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	TGGATAGGATGGCCTTTACG	50.0	50.0	58.1	56.8	482-507 bp	1.0	81.8
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	GGATAGGATGGCCTTTACGT	50.0	50.0	58.1	56.8	481-506 bp	1.0	81.8
trnF-GAA-ndhJ_p5	CTGGTAGAGCAGAGGACTGA	TGGATAGGATGGCCTTTACG	55.0	50.0	57.9	56.8	506-531 bp	1.0	81.0