Hotspot

ycf3-trnS-GGA

LSC · 45325-46201 · 877 bp

Back to Acronema

Pi 0.0372

MarkerSeek score 77.30

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 877
variable_sites: 137
pi: 0.0372
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	CATTTATCCGCGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	47.6	50.0	58.0	58.2	375-1005 bp	1.0	82.5
ycf3-trnS-GGA_p2	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTCAA	47.6	45.0	58.0	57.8	325-955 bp	1.0	82.1
ycf3-trnS-GGA_p3	CATTTATCCGCGATCTAGGCA	AGTTCCAATGCTACGCCTTC	47.6	50.0	58.0	58.3	327-957 bp	1.0	81.6
ycf3-trnS-GGA_p4	CATTTATCCGCGATCTAGGCA	CCCTCGGTAAACAAAAGCCT	47.6	50.0	58.0	58.1	354-984 bp	0.941176	80.6
ycf3-trnS-GGA_p5	CATTTATCCGCGATCTAGGCA	ACCCTCGGTAAACAAAAGCC	47.6	50.0	58.0	58.4	355-985 bp	0.941176	78.2