Hotspot

trnE-UUC-trnT-GGU

LSC · 31613-32337 · 725 bp

Back to Acronema

Pi 0.0473

MarkerSeek score 80.10

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 725
variable_sites: 141
pi: 0.0473
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	GTGCTCTGACCGATTGAACT	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	1050-1450 bp	1.0	91.3
trnE-UUC-trnT-GGU_p2	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	717-1123 bp	1.0	90.6
trnE-UUC-trnT-GGU_p3	GTGCTCTGACCGATTGAACT	CACGAAGCTGCGATCTGATA	50.0	50.0	57.9	57.9	1674-2136 bp	1.0	90.3
trnE-UUC-trnT-GGU_p4	GTGCTCTGACCGATTGAACT	CCGATGACTTACGCCTTACC	50.0	55.0	57.9	58.2	1047-1447 bp	1.0	90.1
trnE-UUC-trnT-GGU_p5	GTGCTCTGACCGATTGAACT	TGGTGATTCCAGAGACGACT	50.0	50.0	57.9	58.1	1246-1620 bp	0.941176	88.4