Hotspot

rps2-rpoC2

LSC · 16696-16926 · 231 bp

Back to Acronema

Pi 0.0453

MarkerSeek score 75.50

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 231
variable_sites: 48
pi: 0.0453
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	AATGAACTCCGGCCTTCATC	TTGGGGGTTCTTGATTGGTG	50.0	50.0	58.0	58.0	889-922 bp	1.0	89.8
rps2-rpoC2_p2	AACATAATGACACCGAGCCC	TTGGGGGTTCTTGATTGGTG	50.0	50.0	58.0	58.0	1119-1152 bp	1.0	89.2
rps2-rpoC2_p3	CCGCATCTCTTTTCGGAAGA	TTGGGGGTTCTTGATTGGTG	50.0	50.0	58.0	58.0	1246-1279 bp	0.882353	81.6
rps2-rpoC2_p4	CCGCATCTCTTTTCGGAAGA	GAGGGTTGGCACGAATGTAT	50.0	50.0	58.0	58.0	1287-1320 bp	0.882353	81.3
rps2-rpoC2_p5	CCGCATCTCTTTTCGGAAGA	GATTCGAGGGATTGGTGCAT	50.0	50.0	58.0	58.0	828-834 bp	0.176471	47.7