Hotspot

atpH-atpI

LSC · 13842-14978 · 1137 bp

Back to Acronema

Pi 0.0417

MarkerSeek score 75.80

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1137
variable_sites: 203
pi: 0.0417
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTCTAATTGGTCAGGAGCGC	45.0	50.0	58.0	58.0	1279-1631 bp	1.0	91.5
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	CTAATTGGTCAGGAGCGCTT	45.0	50.0	58.0	58.0	1277-1629 bp	1.0	91.5
atpH-atpI_p3	ATAACGGAAGCGGCAGAAAT	TGCTTTGGCTTTACTCACGT	45.0	45.0	58.0	58.0	1186-1538 bp	1.0	91.4
atpH-atpI_p4	CTCGCGATACCTTCTACAGC	TTCTAATTGGTCAGGAGCGC	55.0	50.0	58.0	58.0	1369-1721 bp	0.941176	89.1
atpH-atpI_p5	CTCGCGATACCTTCTACAGC	CTAATTGGTCAGGAGCGCTT	55.0	50.0	58.0	58.0	1367-1719 bp	0.941176	89.1