Hotspot

trnH-GUG-psbA

LSC · 287-459 · 173 bp

Back to Acronema

Pi 0.0574

MarkerSeek score 75.60

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 173
variable_sites: 44
pi: 0.0574
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CAATCCACTGCCTTGATCCA	AGCTCCATCTACAAATGGGT	50.0	45.0	57.9	56.2	220-449 bp	1.0	76.5
trnH-GUG-psbA_p2	AATCCACTGCCTTGATCCAC	AGCTCCATCTACAAATGGGT	50.0	45.0	57.9	56.2	219-448 bp	1.0	76.5
trnH-GUG-psbA_p3	ACAATCCACTGCCTTGATCC	AGCTCCATCTACAAATGGGT	50.0	45.0	57.9	56.2	221-450 bp	1.0	76.5
trnH-GUG-psbA_p4	GATCCACTTGGCTACATCCG	AGCTCCATCTACAAATGGGT	55.0	45.0	58.1	56.2	206-435 bp	0.941176	74.2
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	AAGCTCCATCTACAAATGGGT	55.0	42.9	58.1	57.0	207-436 bp	0.941176	72.4