Hotspot

rpl14

LSC · 81705-82073 · 369 bp

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Pi 0

MarkerSeek score 33.90

Primer available yes

Label rpl14

Feature Metadata

label_name: rpl14
feature_type: gene
region: LSC
length_bp: 369
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 33.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14_p1	TGCGTGAGACACAATCTATT	TCCTTCATCCTTTGCATTGA	40.0	40.0	54.8	54.9	486 bp	1.0	40.0
rpl14_p2	TGCGTGAGACACAATCTATT	TCCTTCATCCTTTGCATTGAA	40.0	38.1	54.8	55.7	486 bp	1.0	40.0
rpl14_p3	TGCGTGAGACACAATCTATT	TTCCTTCATCCTTTGCATTGA	40.0	38.1	54.8	55.7	487 bp	1.0	40.0
rpl14_p4	ATATGCGTGAGACACAATCT	TCCTTCATCCTTTGCATTGA	40.0	40.0	54.6	54.9	489 bp	1.0	40.0
rpl14_p5	ATGCGTGAGACACAATCTAT	TCCTTCATCCTTTGCATTGA	40.0	40.0	54.6	54.9	487 bp	1.0	40.0