Hotspot

ndhB

IRa · 143234-145470 · 2237 bp

Back to Acrocomia

Pi 0.000447
MarkerSeek score 57.50
Primer available yes
Label ndhB

Feature Metadata

label_name
ndhB
feature_type
gene
region
IRa
length_bp
2237
variable_sites
1
pi
0.000447
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhB_p1 GTTCATAACCCCAGCCCATT TTCGGAGATTGGATGCAGTT 50.0 45.0 57.8 57.2 2371 bp 1.0 72.5
ndhB_p2 GTTCATAACCCCAGCCCATT TTTCGGAGATTGGATGCAGT 50.0 45.0 57.8 57.2 2372 bp 1.0 72.5
ndhB_p3 TTTGGGCGGAACAGATCTAC TTCGGAGATTGGATGCAGTT 50.0 45.0 57.6 57.2 2342 bp 1.0 70.6
ndhB_p4 TTTGGGCGGAACAGATCTAC TTTCGGAGATTGGATGCAGT 50.0 45.0 57.6 57.2 2343 bp 1.0 70.6
ndhB_p5 TTGGGCGGAACAGATCTACT TTCGGAGATTGGATGCAGTT 50.0 45.0 58.4 57.2 2341 bp 1.0 70.3
ndhB_p1 TTCGGAGATTGGATGCAGTT GTTCATAACCCCAGCCCATT 45.0 50.0 57.2 57.8 2371 bp 1.0 72.5
ndhB_p2 TTTCGGAGATTGGATGCAGT GTTCATAACCCCAGCCCATT 45.0 50.0 57.2 57.8 2372 bp 1.0 72.5
ndhB_p3 TTCGGAGATTGGATGCAGTT TTTGGGCGGAACAGATCTAC 45.0 50.0 57.2 57.6 2342 bp 1.0 70.6
ndhB_p4 TTTCGGAGATTGGATGCAGT TTTGGGCGGAACAGATCTAC 45.0 50.0 57.2 57.6 2343 bp 1.0 70.6
ndhB_p5 TTCGGAGATTGGATGCAGTT TTGGGCGGAACAGATCTACT 45.0 50.0 57.2 58.4 2341 bp 1.0 70.3