Hotspot

ccsA

SSC · 115561-116529 · 969 bp

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Pi 0

MarkerSeek score 30.90

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 969
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 30.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GGCGGCATCCTCTAAAAGAG	ACTCGAACAAGCAAACTTTCG	55.0	42.9	58.4	57.7	1122 bp	1.0	66.1
ccsA_p2	GGCGGCATCCTCTAAAAGAG	CTCGAACAAGCAAACTTTCGT	55.0	42.9	58.4	57.7	1121 bp	1.0	66.1
ccsA_p3	GGCGGCATCCTCTAAAAGAG	TCGAACAAGCAAACTTTCGT	55.0	40.0	58.4	56.5	1120 bp	1.0	64.9
ccsA_p4	GCGGCATCCTCTAAAAGAGA	ACTCGAACAAGCAAACTTTCG	50.0	42.9	57.4	57.7	1121 bp	1.0	64.6
ccsA_p5	GCGGCATCCTCTAAAAGAGA	CTCGAACAAGCAAACTTTCGT	50.0	42.9	57.4	57.7	1120 bp	1.0	64.6