Hotspot

psaC-ndhE

SSC · 118615-119259 · 645 bp

Back to Acrocomia

Pi 0.00164

MarkerSeek score 64.40

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 645
variable_sites: 1
pi: 0.00164
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	ACATCCGTCCCAAGGTATCA	CTGAAGCAGCTATTGGGCTA	50.0	50.0	58.1	57.4	803-839 bp	1.0	77.6
psaC-ndhE_p2	ACATCCGTCCCAAGGTATCA	TGAAGCAGCTATTGGGCTAG	50.0	50.0	58.1	57.4	802-838 bp	1.0	77.6
psaC-ndhE_p3	ACATCCGTCCCAAGGTATCA	TGTTATAGCTGTTGCGGCTG	50.0	50.0	58.1	58.6	823-859 bp	1.0	77.5
psaC-ndhE_p4	ACATCCGTCCCAAGGTATCA	GAAGCAGCTATTGGGCTAGC	50.0	55.0	58.1	58.8	801-837 bp	1.0	76.5
psaC-ndhE_p5	CAATACATCTGTGGGGCAGG	TGAAGCAGCTATTGGGCTAG	55.0	50.0	58.3	57.4	778-814 bp	1.0	76.2