Hotspot

ccsA-ndhD

SSC · 116530-116744 · 215 bp

Back to Acrocomia

Pi 0.014

MarkerSeek score 60.50

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 215
variable_sites: 3
pi: 0.014
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	ACGAATTCGGCACTTATAGCT	CCCGATTTCGTTCTCTCGTT	42.9	50.0	57.5	58.0	404 bp	1.0	68.9
ccsA-ndhD_p2	ACGAATTCGGCACTTATAGCT	ATCCCGATTTCGTTCTCTCG	42.9	50.0	57.5	57.5	406 bp	1.0	65.0
ccsA-ndhD_p3	ACGAATTCGGCACTTATAGC	CCCGATTTCGTTCTCTCGTT	45.0	50.0	56.0	58.0	404 bp	1.0	64.8
ccsA-ndhD_p4	ACGAATTCGGCACTTATAGCT	TCAGTTGACAGGGTACAAGC	42.9	50.0	57.5	57.5	383 bp	1.0	64.6
ccsA-ndhD_p5	ACGAATTCGGCACTTATAGCT	CAGTTGACAGGGTACAAGCT	42.9	50.0	57.5	57.4	382 bp	1.0	64.6