Hotspot

trnD-GUC-trnY-GUA

LSC · 30904-31334 · 431 bp

Back to Acrocomia

Pi 0.00232
MarkerSeek score 56.60
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
431
variable_sites
1
pi
0.00232
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 GGATCCTAGTTCGGGACTGA AATTCTTCCTGGGTCGATGC 55.0 50.0 57.9 58.0 610 bp 1.0 79.2
trnD-GUC-trnY-GUA_p2 GTGCTCTGACCGATTGAACT AATTCTTCCTGGGTCGATGC 50.0 50.0 57.9 58.0 553 bp 1.0 79.1
trnD-GUC-trnY-GUA_p3 GGATCCTAGTTCGGGACTGA GGGGACGGACTGTAAATTCG 55.0 55.0 57.9 58.4 577 bp 1.0 76.8
trnD-GUC-trnY-GUA_p4 ATCGGATCCTAGTTCGGGAC AATTCTTCCTGGGTCGATGC 55.0 50.0 58.4 58.0 613 bp 1.0 76.7
trnD-GUC-trnY-GUA_p5 CATCGGATCCTAGTTCGGGA AATTCTTCCTGGGTCGATGC 55.0 50.0 58.4 58.0 614 bp 1.0 76.7