Hotspot

ycf1

SSC · 123624-129365 · 5742 bp

Back to Acorus

Pi 0.00978
MarkerSeek score 36.70
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5742
variable_sites
96
pi
0.00978
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
36.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 TCCGTTCATCTTCCTCCCTT AGCCCAGGTTACGAAAACTC 50.0 50.0 58.0 57.8 1444-1450 bp 1.0 80.3
ycf1_p2 TCCGTTCATCTTCCTCCCTT GCCCAGGTTACGAAAACTCT 50.0 50.0 58.0 57.8 1443-1449 bp 1.0 80.3
ycf1_p3 TACACGTCTGCCTTTTCTCG AGCCCAGGTTACGAAAACTC 50.0 50.0 57.9 57.8 1044-1050 bp 1.0 80.1
ycf1_p4 TACACGTCTGCCTTTTCTCG GCCCAGGTTACGAAAACTCT 50.0 50.0 57.9 57.8 1043-1049 bp 1.0 80.1
ycf1_p5 TAGCGCGTATGGATCCATTG GCCCAGGTTACGAAAACTCT 50.0 50.0 57.9 57.8 1212-1218 bp 1.0 80.1