Hotspot

ycf1-tRNA-Asn (GUU)

IRa · 129366-129968 · 603 bp

Back to Acorus

Pi 0.0058
MarkerSeek score 55.00
Primer available yes
Label ycf1-tRNA-Asn (GUU)

Feature Metadata

label_name
ycf1-tRNA-Asn (GUU)
feature_type
igs
region
IRa
length_bp
603
variable_sites
6
pi
0.0058
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.79
markerseek_score
55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1-tRNA-Asn (GUU)_p1 ATAGTCCGACCACAACGACT AGGATTCGAACCTACGACCA 50.0 50.0 58.5 58.2 619-747 bp 1.0 79.8
ycf1-tRNA-Asn (GUU)_p2 CCGACCACAACGACTGAATT AGGATTCGAACCTACGACCA 50.0 50.0 58.5 58.2 614-742 bp 1.0 79.5
ycf1-tRNA-Asn (GUU)_p3 ATAGTCCGACCACAACGACT AATCAGTTAACAGCCGACCG 50.0 50.0 58.5 58.3 600-728 bp 1.0 78.8
ycf1-tRNA-Asn (GUU)_p4 CCGACCACAACGACTGAATT AATCAGTTAACAGCCGACCG 50.0 50.0 58.5 58.3 595-723 bp 1.0 78.6
ycf1-tRNA-Asn (GUU)_p5 ATAGTCCGACCACAACGACT CCCCAAGTAGGATTCGAACC 50.0 55.0 58.5 57.7 627-755 bp 1.0 78.4