Hotspot

ndhF-rpl32

SSC · 112250-113100 · 851 bp

Back to Acorus

Pi 0.017
MarkerSeek score 63.10
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
851
variable_sites
23
pi
0.017
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.96
markerseek_score
63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGAAGGAAAGGTATTATCCATGC GTACGTTTCTTTGGAACCGC 43.5 50.0 57.0 57.7 891-918 bp 1.0 51.7
ndhF-rpl32_p2 GGAAGGAAAGGTATTATCCATGC GCTTTTACCGCGGCAAAATA 43.5 45.0 57.0 57.7 960-987 bp 1.0 51.6
ndhF-rpl32_p3 GGAAGGAAAGGTATTATCCATGC CGCGGCAAAATATCCCTTTT 43.5 45.0 57.0 57.4 952-979 bp 1.0 49.1
ndhF-rpl32_p4 GGAAGGAAAGGTATTATCCATGC TTACCGCGGCAAAATATCCC 43.5 50.0 57.0 58.7 956-983 bp 1.0 48.2
ndhF-rpl32_p5 GGAAGGAAAGGTATTATCCATGC AGAAAAAGCTTTTACCGCGG 43.5 45.0 57.0 57.3 967-994 bp 1.0 48.1