Hotspot

psbT-psbN

LSC · 74014-74072 · 59 bp

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Pi 0.0452
MarkerSeek score 64.70
Primer available yes
Label psbT-psbN

Feature Metadata

label_name
psbT-psbN
feature_type
igs
region
LSC
length_bp
59
variable_sites
5
pi
0.0452
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbT-psbN_p1 ATCTTCTTTCGAGAACCGCC TTCGAGGAACACGGAGACTA 50.0 50.0 58.0 57.8 128 bp 1.0 81.5
psbT-psbN_p2 TTCTTTCGAGAACCGCCAAA TTCGAGGAACACGGAGACTA 45.0 50.0 58.0 57.8 125 bp 1.0 81.3
psbT-psbN_p3 ATCTTCTTTCGAGAACCGCC TCGAGGAACACGGAGACTAG 50.0 55.0 58.0 58.3 127 bp 1.0 80.9
psbT-psbN_p4 TTCTTTCGAGAACCGCCAAA TCGAGGAACACGGAGACTAG 45.0 55.0 58.0 58.3 124 bp 1.0 80.8
psbT-psbN_p5 ATCTTCTTTCGAGAACCGCC CATTCGAGGAACACGGAGAC 50.0 55.0 58.0 58.4 130 bp 1.0 80.0