Hotspot

rbcL-psaI

LSC · 58157-59415 · 1259 bp

Back to Acorus

Pi 0.0116
MarkerSeek score 52.20
Primer available yes
Label rbcL-psaI

Feature Metadata

label_name
rbcL-psaI
feature_type
igs
region
LSC
length_bp
1259
variable_sites
24
pi
0.0116
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-psaI_p1 ACTACAGTTCGGTGGAGGAA TTGTTTTTCGAGACCCCGTT 50.0 45.0 58.0 58.0 2043-2050 bp 1.0 81.4
rbcL-psaI_p2 TCTTGCTCGCGAAAGTACTC TTGTTTTTCGAGACCCCGTT 50.0 45.0 58.0 58.0 1932-1939 bp 1.0 81.4
rbcL-psaI_p3 AATGAGGGACGTGATCTTGC TTGTTTTTCGAGACCCCGTT 50.0 45.0 58.0 58.0 1946-1953 bp 1.0 81.3
rbcL-psaI_p4 CTTGACCGAGATCTTTGGGG TTGTTTTTCGAGACCCCGTT 55.0 45.0 58.0 58.0 2073-2080 bp 1.0 81.2
rbcL-psaI_p5 TTGAATGCTACTGCGGGTAC TTGTTTTTCGAGACCCCGTT 50.0 45.0 58.0 58.0 2529 bp 0.5 59.6