Hotspot

matK-tRNA-Lys (UUU)

LSC · 3544-4250 · 707 bp

Back to Acorus

Pi 0.00872
MarkerSeek score 55.10
Primer available yes
Label matK-tRNA-Lys (UUU)

Feature Metadata

label_name
matK-tRNA-Lys (UUU)
feature_type
igs
region
LSC
length_bp
707
variable_sites
10
pi
0.00872
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-tRNA-Lys (UUU)_p1 AGTGCGTAGATGTACTCCTGA TCTGGGTTGCTAACTCAACG 47.6 50.0 58.0 57.8 839 bp 1.0 71.6
matK-tRNA-Lys (UUU)_p2 AGTGCGTAGATGTACTCCTGA AACGGTAGAGTACTCGGCTT 47.6 50.0 58.0 58.2 823 bp 1.0 71.6
matK-tRNA-Lys (UUU)_p3 AGTGCGTAGATGTACTCCTGA ACGGTAGAGTACTCGGCTTT 47.6 50.0 58.0 58.2 822 bp 1.0 71.6
matK-tRNA-Lys (UUU)_p4 GACGAGACCCATCTTTTTCGA TCTGGGTTGCTAACTCAACG 47.6 50.0 58.0 57.8 790 bp 1.0 71.3
matK-tRNA-Lys (UUU)_p5 GACGAGACCCATCTTTTTCGA ACGGTAGAGTACTCGGCTTT 47.6 50.0 58.0 58.2 773 bp 1.0 71.3