Hotspot

tRNA-His (GUG)-psbA

LSC · 79-463 · 385 bp

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Pi 0.0652
MarkerSeek score 57.50
Primer available yes
Label tRNA-His (GUG)-psbA

Feature Metadata

label_name
tRNA-His (GUG)-psbA
feature_type
igs
region
LSC
length_bp
385
variable_sites
11
pi
0.0652
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.35
markerseek_score
57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-His (GUG)-psbA_p1 CTGCCTTAATCCACTTGGCT TAATATCGTCGCTGCTCACG 50.0 50.0 57.9 58.0 742 bp 0.25 48.7
tRNA-His (GUG)-psbA_p2 CTGCCTTAATCCACTTGGCT TCCATTCCACATGTTAGGCG 50.0 50.0 57.9 58.0 895 bp 0.25 48.6
tRNA-His (GUG)-psbA_p3 ACTGCCTTAATCCACTTGGC TAATATCGTCGCTGCTCACG 50.0 50.0 58.2 58.0 743 bp 0.25 48.5
tRNA-His (GUG)-psbA_p4 ACTGCCTTAATCCACTTGGC TCCATTCCACATGTTAGGCG 50.0 50.0 58.2 58.0 896 bp 0.25 48.4
tRNA-His (GUG)-psbA_p5 CTGCCTTAATCCACTTGGCT GTTGACAGTCAAGGTCGTGT 50.0 50.0 57.9 58.1 561 bp 0.25 48.4