Hotspot

ycf1

SSC · 125499-130841 · 5343 bp

Back to Aconitum

Pi 0.012

MarkerSeek score 51.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5343
variable_sites: 319
pi: 0.012
unique_haplotype_count: 44
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGGCTATTATCAACAAAGGCA	GAGTAAGGGCGCGGAATTAA	38.1	50.0	55.5	58.1	221-251 bp	0.981132	59.2
ycf1_p2	TGGCTATTATCAACAAAGGCA	GGCGCGGAATTAAGGACTTA	38.1	50.0	55.5	58.1	214-244 bp	0.981132	59.2
ycf1_p3	TGGCTATTATCAACAAAGGCA	AGTAAGGGCGCGGAATTAAG	38.1	50.0	55.5	58.1	220-250 bp	0.962264	58.4
ycf1_p4	TGGCTATTATCAACAAAGGCAA	AGTAAGGGCGCGGAATTAAG	36.4	50.0	56.3	58.1	220-250 bp	0.962264	56.6
ycf1_p5	TTGGCTATTATCAACAAAGGCA	AGTAAGGGCGCGGAATTAAG	36.4	50.0	56.3	58.1	221-251 bp	0.962264	56.6