Hotspot

ndhF

SSC · 112676-114919 · 2244 bp

Back to Aconitum

Pi 0.0057

MarkerSeek score 41.30

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2244
variable_sites: 88
pi: 0.0057
unique_haplotype_count: 28
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CCTTCCCCTTCCCTTTGTTC	AGAGCTGGTGAATCGGAAAC	55.0	50.0	58.1	57.9	2640-2984 bp	1.0	87.9
ndhF_p2	ACAGGAACAAGAGGGATCCA	AGAGCTGGTGAATCGGAAAC	50.0	50.0	58.0	57.9	2671-2995 bp	0.90566	84.3
ndhF_p3	AAGAGGGATCCACCGAAGAA	AGAGCTGGTGAATCGGAAAC	50.0	50.0	58.0	57.9	2663-2987 bp	0.90566	84.3
ndhF_p4	ACAGGAACAAGAGGGATCCA	GAGCTGGTGAATCGGAAACA	50.0	50.0	58.0	58.2	2670-2994 bp	0.90566	83.6
ndhF_p5	AAGAGGGATCCACCGAAGAA	GAGCTGGTGAATCGGAAACA	50.0	50.0	58.0	58.2	2662-2986 bp	0.90566	83.6