Hotspot

ndhF-trnL-UAG

SSC · 114920-115787 · 868 bp

Back to Aconitum

Pi 0.0137
MarkerSeek score 65.10
Primer available yes
Label ndhF-trnL-UAG

Feature Metadata

label_name
ndhF-trnL-UAG
feature_type
igs
region
SSC
length_bp
868
variable_sites
80
pi
0.0137
unique_haplotype_count
35
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-trnL-UAG_p1 ACGAAAGGTATGATCCACGC AGCGTGTCTACCGATTTCAC 50.0 50.0 58.1 58.0 925-968 bp 1.0 90.2
ndhF-trnL-UAG_p2 CGAAAGGTATGATCCACGCA AGCGTGTCTACCGATTTCAC 50.0 50.0 58.1 58.0 924-967 bp 1.0 90.2
ndhF-trnL-UAG_p3 ACGAAAGGTATGATCCACGC CAGCGTGTCTACCGATTTCA 50.0 50.0 58.1 58.0 926-969 bp 1.0 90.2
ndhF-trnL-UAG_p4 GAGAAGCCCCAACCCTATTG AGCGTGTCTACCGATTTCAC 55.0 50.0 57.9 58.0 965-1008 bp 0.981132 88.8
ndhF-trnL-UAG_p5 GAGAAGCCCCAACCCTATTG CAGCGTGTCTACCGATTTCA 55.0 50.0 57.9 58.0 966-1009 bp 0.981132 88.7