Hotspot

psbT-psbN

LSC · 76053-76138 · 86 bp

Back to Aconitum

Pi 0.0266

MarkerSeek score 60.80

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 86
variable_sites: 6
pi: 0.0266
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	AGGTTCCGACTAAAAAGGCG	GGGTACGCCTTATATACCGC	50.0	55.0	58.2	57.7	172-189 bp	1.0	83.2
psbT-psbN_p2	GGTTCCGACTAAAAAGGCGA	GGGTACGCCTTATATACCGC	50.0	55.0	58.2	57.7	171-188 bp	1.0	83.1
psbT-psbN_p3	AGGTTCCGACTAAAAAGGCG	GTTTTACCGGGTACGCCTTA	50.0	50.0	58.2	57.6	180-197 bp	1.0	82.3
psbT-psbN_p4	GGTTCCGACTAAAAAGGCGA	GTTTTACCGGGTACGCCTTA	50.0	50.0	58.2	57.6	179-196 bp	1.0	82.3
psbT-psbN_p5	AGGTTCCGACTAAAAAGGCG	TAAGTTTTACCGGGTACGCC	50.0	50.0	58.2	57.6	183-200 bp	0.981132	81.1