Hotspot

trnP-UGG-psaJ

LSC · 68016-68633 · 618 bp

Back to Aconitum

Pi 0.0163

MarkerSeek score 60.20

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 618
variable_sites: 53
pi: 0.0163
unique_haplotype_count: 31
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	CGGGTGCCACAGAAAGATAT	50.0	50.0	58.1	57.7	510-749 bp	1.0	86.6
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	CGGGTGCCACAGAAAGATAT	50.0	50.0	58.1	57.7	509-748 bp	1.0	86.6
trnP-UGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	CGGGTGCCACAGAAAGATAT	50.0	50.0	58.1	57.7	521-760 bp	1.0	86.5
trnP-UGG-psaJ_p4	CAGGATTTGAACCCGTGACA	CGGGTGCCACAGAAAGATAT	50.0	50.0	58.1	57.7	520-759 bp	1.0	86.5
trnP-UGG-psaJ_p5	TGACAGGATTTGAACCCGTG	CGGGTGCCACAGAAAGATAT	50.0	50.0	58.1	57.7	523-754 bp	0.981132	85.6