Hotspot

ndhC-trnV-UAC

LSC · 52993-53403 · 411 bp

Back to Aconitum

Pi 0.0265

MarkerSeek score 64.60

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 411
variable_sites: 50
pi: 0.0265
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGGCCAAAACTCCGGAAATT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	59.0	57.8	405-1545 bp	1.0	79.9
ndhC-trnV-UAC_p2	GGGCCAAAACTCCGGAAAT	AGAAGGTCTACGGTTCGAGT	52.6	50.0	58.3	57.8	405-1545 bp	1.0	77.4
ndhC-trnV-UAC_p3	GGGCCAAAACTCCGGAAATT	GAAGGTCTACGGTTCGAGTC	50.0	55.0	59.0	57.5	404-1544 bp	1.0	77.2
ndhC-trnV-UAC_p4	GGGCCAAAACTCCGGAAATT	GTTCGAGTCCGTATAGCCCT	50.0	55.0	59.0	58.7	393-1533 bp	1.0	76.1
ndhC-trnV-UAC_p5	GGGCCAAAACTCCGGAAATT	AAGGTCTACGGTTCGAGTCC	50.0	55.0	59.0	58.8	403-1543 bp	1.0	74.9