Hotspot

ndhK-ndhC

LSC · 52564-52629 · 66 bp

Back to Aconitum

Pi 0.0294

MarkerSeek score 62.30

Primer available yes

Label ndhK-ndhC

Feature Metadata

label_name: ndhK-ndhC
feature_type: igs
region: LSC
length_bp: 66
variable_sites: 12
pi: 0.0294
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK-ndhC_p1	AGAGAAGCGGCCATAAACTG	CATGGCGAAAAGGAGCATTG	50.0	50.0	58.0	58.1	204-217 bp	1.0	87.4
ndhK-ndhC_p2	ACCATAGAGAAGCGGCCATA	CATGGCGAAAAGGAGCATTG	50.0	50.0	58.3	58.1	209-222 bp	1.0	85.2
ndhK-ndhC_p3	AGAGAAGCGGCCATAAACTG	GGGCGATGAGTTTCGATGTA	50.0	50.0	58.0	57.8	291-304 bp	1.0	83.9
ndhK-ndhC_p4	ACCATAGAGAAGCGGCCATA	GGGCGATGAGTTTCGATGTA	50.0	50.0	58.3	57.8	296-309 bp	1.0	81.8
ndhK-ndhC_p5	CCATAGAGAAGCGGCCATAA	CATGGCGAAAAGGAGCATTG	50.0	50.0	57.2	58.1	208-221 bp	1.0	80.8