Pi
0.016
MarkerSeek score
61.30
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 735-789 bp | 1.0 | 87.7 |
| trnT-UGU-trnL-UAA_p2 | GCGATGCTCTAACCTCTGAG | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 57.9 | 58.1 | 743-797 bp | 1.0 | 87.2 |
| trnT-UGU-trnL-UAA_p3 | ACAAATAGACAAAGCCGGCT | ACCAATTTCGCCATATCCCC | 45.0 | 50.0 | 57.8 | 58.0 | 790-844 bp | 1.0 | 86.9 |
| trnT-UGU-trnL-UAA_p4 | GGAATCGAACCGATGACCAT | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.8 | 58.0 | 767-821 bp | 1.0 | 86.7 |
| trnT-UGU-trnL-UAA_p5 | ACAAATAGACAAAGCCGGCT | TAGCGTCTACCAATTTCGCC | 45.0 | 50.0 | 57.8 | 58.1 | 798-852 bp | 1.0 | 86.3 |