Hotspot

trnE-UUC-trnT-GGU

LSC · 32374-33171 · 798 bp

Back to Aconitum

Pi 0.0231

MarkerSeek score 75.50

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 798
variable_sites: 95
pi: 0.0231
unique_haplotype_count: 30
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	TACACGATCAACTACCCCCA	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	932-1011 bp	1.0	88.9
trnE-UUC-trnT-GGU_p2	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	895-974 bp	1.0	88.8
trnE-UUC-trnT-GGU_p3	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	874-953 bp	1.0	88.7
trnE-UUC-trnT-GGU_p4	TACACGATCAACTACCCCCA	TACCGCTGAGTTAAAAGGGC	50.0	50.0	57.8	57.9	897-976 bp	1.0	88.3
trnE-UUC-trnT-GGU_p5	GCTGCCTCCTTGAAAGAGAG	TACCGCTGAGTTAAAAGGGC	55.0	50.0	58.3	57.9	860-939 bp	1.0	88.2