Hotspot

rpl16

LSC · 80305-81784 · 1480 bp

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Pi 0.0131

MarkerSeek score 53.30

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1480
variable_sites: 29
pi: 0.0131
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	ACCGTTGCTACGTTTGAGTT	GCAGAATTCATAGCCGGACA	45.0	50.0	58.1	58.0	2230-2237 bp	1.0	81.1
rpl16_p2	ACCGTTGCTACGTTTGAGTT	TGCAGAATTCATAGCCGGAC	45.0	50.0	58.1	58.0	2231-2238 bp	1.0	81.1
rpl16_p3	GCTCCGCTATTATCGGCTAC	GCAGAATTCATAGCCGGACA	55.0	50.0	58.1	58.0	2055-2062 bp	1.0	81.0
rpl16_p4	GCTCCGCTATTATCGGCTAC	TGCAGAATTCATAGCCGGAC	55.0	50.0	58.1	58.0	2056-2063 bp	1.0	81.0
rpl16_p5	ACCGTTGCTACGTTTGAGTT	TCGAGGGAATTGGACGGATA	45.0	50.0	58.1	57.9	2442-2449 bp	1.0	80.8