Hotspot

trnG-UCC-trnS-GCU

LSC · 53094-53896 · 803 bp

Back to Acmispon

Pi 0.0208

MarkerSeek score 60.40

Primer available yes

Label trnG-UCC-trnS-GCU

Feature Metadata

label_name: trnG-UCC-trnS-GCU
feature_type: igs
region: LSC
length_bp: 803
variable_sites: 25
pi: 0.0208
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnS-GCU_p1	ACGAATCGCACTTTTACCACT	CAATCCGACGCTTTAGTCCA	42.9	50.0	58.2	58.0	872-885 bp	1.0	72.5
trnG-UCC-trnS-GCU_p2	ACGAATCGCACTTTTACCACT	AATCCGACGCTTTAGTCCAC	42.9	50.0	58.2	58.0	871-884 bp	1.0	72.5
trnG-UCC-trnS-GCU_p3	ACGAATCGCACTTTTACCAC	CAATCCGACGCTTTAGTCCA	45.0	50.0	56.8	58.0	872-885 bp	1.0	72.5
trnG-UCC-trnS-GCU_p4	ACGAATCGCACTTTTACCAC	AATCCGACGCTTTAGTCCAC	45.0	50.0	56.8	58.0	871-884 bp	1.0	72.5
trnG-UCC-trnS-GCU_p5	TCCATAGAACGAATCGCACT	CAATCCGACGCTTTAGTCCA	45.0	50.0	56.8	58.0	880-893 bp	1.0	72.4