Hotspot

trnR-UCU-trnG-UCC

LSC · 52092-52297 · 206 bp

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Pi 0.0324
MarkerSeek score 72.50
Primer available yes
Label trnR-UCU-trnG-UCC

Feature Metadata

label_name
trnR-UCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
206
variable_sites
10
pi
0.0324
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-trnG-UCC_p1 TGCGCCCAATAGGATTTGAA AGCCTTCCAAGCTAACGATG 45.0 50.0 57.9 58.0 322-372 bp 1.0 84.2
trnR-UCU-trnG-UCC_p2 TTGCGCCCAATAGGATTTGA AGCCTTCCAAGCTAACGATG 45.0 50.0 57.9 58.0 323-373 bp 1.0 84.2
trnR-UCU-trnG-UCC_p3 TGCGCCCAATAGGATTTGAA ACCCTTAGCCTTCCAAGCTA 45.0 50.0 57.9 58.0 328-378 bp 1.0 84.1
trnR-UCU-trnG-UCC_p4 TTGCGCCCAATAGGATTTGA ACCCTTAGCCTTCCAAGCTA 45.0 50.0 57.9 58.0 329-379 bp 1.0 84.0
trnR-UCU-trnG-UCC_p5 GCGCCCAATAGGATTTGAAC AGCCTTCCAAGCTAACGATG 50.0 50.0 57.5 58.0 321-371 bp 1.0 81.3