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psbM-petN

LSC · 31841-32665 · 825 bp

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Pi 0.023

MarkerSeek score 61.80

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 825
variable_sites: 28
pi: 0.023
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	GCTACTGCGCTCTTCATTCT	TGAAATGGAGGGGGCAAAAA	50.0	45.0	58.1	57.9	1406-1433 bp	1.0	83.4
psbM-petN_p2	TTACAGTGCAACGGTTGGAA	TGAAATGGAGGGGGCAAAAA	45.0	45.0	58.0	57.9	2011-2036 bp	1.0	83.0
psbM-petN_p3	TTACAGTGCAACGGTTGGAA	TAGTAAGTCTTGCCTGGGCT	45.0	50.0	58.0	58.0	1594-1607 bp	1.0	82.5
psbM-petN_p4	GCTACTGCGCTCTTCATTCT	TAGTAAGTCTTGCCTGGGCT	50.0	50.0	58.1	58.0	989-1005 bp	1.0	82.5
psbM-petN_p5	TTGCTACTGCGCTCTTCATT	TAGTAAGTCTTGCCTGGGCT	45.0	50.0	57.9	58.0	991-1007 bp	1.0	82.2