Hotspot

trnT-UGU-rps4

LSC · 14788-15107 · 320 bp

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Pi 0.0187

MarkerSeek score 67.60

Primer available yes

Label trnT-UGU-rps4

Feature Metadata

label_name: trnT-UGU-rps4
feature_type: igs
region: LSC
length_bp: 320
variable_sites: 9
pi: 0.0187
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-rps4_p1	TGATATTCCTGTGCCCATGC	ATGGAGCGCGATTGATTTCT	50.0	45.0	58.0	58.0	543-551 bp	1.0	82.0
trnT-UGU-rps4_p2	CTCAGAGGTTAGAGCATCGC	ATGGAGCGCGATTGATTTCT	55.0	45.0	57.9	58.0	491-499 bp	1.0	81.4
trnT-UGU-rps4_p3	ATGGTCATCGGTTCGATTCC	ATGGAGCGCGATTGATTTCT	50.0	45.0	57.8	58.0	459-467 bp	1.0	80.7
trnT-UGU-rps4_p4	TGATATTCCTGTGCCCATGC	CCCAGACGGCGTATCTTTTT	50.0	50.0	58.0	58.3	474-482 bp	1.0	80.6
trnT-UGU-rps4_p5	TGATATTCCTGTGCCCATGC	AAGTTCGCTTCCGACTGTAG	50.0	50.0	58.0	57.7	523-531 bp	1.0	79.7