Hotspot

atpE-trnM-CAU

LSC · 9218-9365 · 148 bp

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Pi 0.0506

MarkerSeek score 77.30

Primer available yes

Label atpE-trnM-CAU

Feature Metadata

label_name: atpE-trnM-CAU
feature_type: igs
region: LSC
length_bp: 148
variable_sites: 11
pi: 0.0506
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpE-trnM-CAU_p1	CAGACGAGCTAGAACACGAG	TATTGCTTTCATACGGCGGG	55.0	50.0	57.8	58.4	239-243 bp	1.0	80.4
atpE-trnM-CAU_p2	CTCAGACGAGCTAGAACACG	TATTGCTTTCATACGGCGGG	55.0	50.0	57.8	58.4	241-245 bp	1.0	80.3
atpE-trnM-CAU_p3	AGACGAGCTAGAACACGAGT	TATTGCTTTCATACGGCGGG	50.0	50.0	58.2	58.4	238-242 bp	1.0	80.2
atpE-trnM-CAU_p4	TCAGACGAGCTAGAACACGA	TATTGCTTTCATACGGCGGG	50.0	50.0	58.2	58.4	240-244 bp	1.0	80.1
atpE-trnM-CAU_p5	GGCTAAGGGCAAGAGACAAA	TATTGCTTTCATACGGCGGG	50.0	50.0	57.8	58.4	281-285 bp	1.0	79.4