Hotspot

trnK-UUU-rbcL

LSC · 4389-5091 · 703 bp

Back to Acmispon

Pi 0.0155

MarkerSeek score 64.10

Primer available yes

Label trnK-UUU-rbcL

Feature Metadata

label_name: trnK-UUU-rbcL
feature_type: igs
region: LSC
length_bp: 703
variable_sites: 15
pi: 0.0155
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rbcL_p1	AAAGCCGAGTACTCTACCGT	AGTCCTGAATTAGCTGCTGC	50.0	50.0	58.2	58.0	790-812 bp	1.0	83.9
trnK-UUU-rbcL_p2	AAGCCGAGTACTCTACCGTT	AGTCCTGAATTAGCTGCTGC	50.0	50.0	58.2	58.0	789-811 bp	1.0	83.9
trnK-UUU-rbcL_p3	TACCGTTGAGTTAGCAACCC	AGTCCTGAATTAGCTGCTGC	50.0	50.0	57.5	58.0	776-798 bp	1.0	81.6
trnK-UUU-rbcL_p4	AAAGCCGAGTACTCTACCGT	GCTGCTGCTTGTGAAGTATG	50.0	50.0	58.2	57.5	778-800 bp	1.0	79.9
trnK-UUU-rbcL_p5	AAGCCGAGTACTCTACCGTT	GCTGCTGCTTGTGAAGTATG	50.0	50.0	58.2	57.5	777-799 bp	1.0	79.9