Hotspot

matK-trnK-UUU

LSC · 3233-3983 · 751 bp

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Pi 0
MarkerSeek score 40.00
Primer available yes
Label matK-trnK-UUU

Feature Metadata

label_name
matK-trnK-UUU
feature_type
igs
region
LSC
length_bp
751
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-trnK-UUU_p1 TACAGGAAGTGTTGTTGCCG TGGGTTGCTAACTCAATGGT 50.0 45.0 58.4 57.0 848 bp 1.0 69.0
matK-trnK-UUU_p2 TACAGGAAGTGTTGTTGCCG TCAATGGTAGAGTACTCGGCT 50.0 47.6 58.4 58.0 836 bp 1.0 68.6
matK-trnK-UUU_p3 GGAAGTGTTGTTGCCGAGAT TGGGTTGCTAACTCAATGGT 50.0 45.0 58.5 57.0 844 bp 1.0 68.4
matK-trnK-UUU_p4 TACAGGAAGTGTTGTTGCCG TCTGGGTTGCTAACTCAATGG 50.0 47.6 58.4 57.9 850 bp 1.0 68.1
matK-trnK-UUU_p5 GGAAGTGTTGTTGCCGAGAT TCAATGGTAGAGTACTCGGCT 50.0 47.6 58.5 58.0 832 bp 1.0 68.1