Hotspot

rpl22

LSC · 82789-83238 · 450 bp

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Pi 0

MarkerSeek score 23.20

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 450
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 23.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	GTGCGAACCAAAAGGAATGG	CAAATTGGGGGAATTCGTGC	50.0	50.0	57.9	58.0	723 bp	1.0	79.9
rpl22_p2	GTGCGAACCAAAAGGAATGG	GCAAATTGGGGGAATTCGTG	50.0	50.0	57.9	58.0	724 bp	1.0	79.9
rpl22_p3	CAGAACTTGAGCCTGAGTCC	CAAATTGGGGGAATTCGTGC	55.0	50.0	57.9	58.0	891 bp	1.0	79.6
rpl22_p4	CAGAACTTGAGCCTGAGTCC	GCAAATTGGGGGAATTCGTG	55.0	50.0	57.9	58.0	892 bp	1.0	79.6
rpl22_p5	GTGCGAACCAAAAGGAATGG	GTTATCCTGCGCTTGGAAGA	50.0	50.0	57.9	58.0	1264 bp	1.0	79.5