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rpl22-rps19

IRb · 83239-83302 · 64 bp

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Pi 0.0417

MarkerSeek score 66.60

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: IRb
length_bp: 64
variable_sites: 4
pi: 0.0417
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	CGAGTTTCCTTAGCCCACTC	CAAATTGGGGGAATTCGTGC	55.0	50.0	58.0	58.0	213 bp	1.0	81.3
rpl22-rps19_p2	CGAGTTTCCTTAGCCCACTC	GCAAATTGGGGGAATTCGTG	55.0	50.0	58.0	58.0	214 bp	1.0	81.3
rpl22-rps19_p3	TCCTTGCGAGTTTCCTTAGC	CAAATTGGGGGAATTCGTGC	50.0	50.0	57.9	58.0	219 bp	1.0	80.6
rpl22-rps19_p4	TCCTTGCGAGTTTCCTTAGC	GCAAATTGGGGGAATTCGTG	50.0	50.0	57.9	58.0	220 bp	1.0	80.6
rpl22-rps19_p5	CGAGTTTCCTTAGCCCACTC	ATGGTAGGTCGCAAATTGGG	55.0	50.0	58.0	58.2	224 bp	1.0	79.4