Hotspot

rps16-trnQ-UUG

LSC · 5617-7147 · 1531 bp

Back to Acidosasa

Pi 0.000435
MarkerSeek score 46.50
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1531
variable_sites
1
pi
0.000435
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1605 bp 1.0 78.3
rps16-trnQ-UUG_p2 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 1616 bp 1.0 77.8
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA TAAAACCAGAAATGGGGCGT 45.0 45.0 58.1 57.7 1665 bp 1.0 76.6
rps16-trnQ-UUG_p4 TCATGTCCTTCAAGTCGCAC TAAAACCAGAAATGGGGCGT 50.0 45.0 58.2 57.7 1676 bp 1.0 76.0
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA TTCGGAGGTTCGAATCCTTC 45.0 50.0 58.1 57.3 1609 bp 1.0 73.6