Hotspot

rpoC2

LSC · 16141-20250 · 4110 bp

Back to Achyranthes

Pi 0.000649

MarkerSeek score 54.30

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4110
variable_sites: 4
pi: 0.000649
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	ACGTACGAACAGTTCCTTGG	AGCCACTCGAGAAATTTCGG	50.0	50.0	57.9	58.3	320 bp	1.0	77.2
rpoC2_p2	CGTACGAACAGTTCCTTGGT	AGCCACTCGAGAAATTTCGG	50.0	50.0	57.9	58.3	319 bp	1.0	77.2
rpoC2_p3	ACGTACGAACAGTTCCTTGG	GCCACTCGAGAAATTTCGGA	50.0	50.0	57.9	58.3	319 bp	1.0	77.1
rpoC2_p4	CGTACGAACAGTTCCTTGGT	GCCACTCGAGAAATTTCGGA	50.0	50.0	57.9	58.3	318 bp	1.0	77.1
rpoC2_p5	CCCAACAACGTACGAACAGT	AGCCACTCGAGAAATTTCGG	50.0	50.0	58.4	58.3	327 bp	1.0	74.9