Hotspot

atpF

LSC · 11550-12892 · 1343 bp

Back to Achyranthes

Pi 0.000994

MarkerSeek score 61.80

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1343
variable_sites: 2
pi: 0.000994
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	TCGTCTGCTCGAATGGTTAC	GCAAAGAAGTCAAATAATACAACGG	50.0	36.0	57.7	57.5	1444-1497 bp	1.0	42.7
atpF_p2	GTCTGCTCGAATGGTTACCA	GCAAAGAAGTCAAATAATACAACGG	50.0	36.0	57.6	57.5	1442-1495 bp	1.0	42.7
atpF_p3	TTTCGTCTGCTCGAATGGTT	GCAAAGAAGTCAAATAATACAACGG	45.0	36.0	57.8	57.5	1446-1499 bp	1.0	42.6
atpF_p4	ATTTCGTCTGCTCGAATGGT	GCAAAGAAGTCAAATAATACAACGG	45.0	36.0	57.6	57.5	1447-1500 bp	1.0	42.6
atpF_p5	TTTCGTCTGCTCGAATGGTT	AGAAGTAGCAAAGAAGTCAA	45.0	35.0	57.8	52.0	1453-1506 bp	1.0	42.6