Hotspot

ndhH-ycf1

SSC · 121634-122303 · 670 bp

Back to Achyranthes

Pi 0.00199
MarkerSeek score 33.10
Primer available yes
Label ndhH-ycf1

Feature Metadata

label_name
ndhH-ycf1
feature_type
igs
region
SSC
length_bp
670
variable_sites
2
pi
0.00199
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
33.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhH-ycf1_p1 CGGTAGCTACCCCTCCAATA TACCGGGAAATTGGGCAAAA 55.0 45.0 58.0 58.0 1387-1388 bp 1.0 80.0
ndhH-ycf1_p2 TCTGTGCACCAATATCTGCC TACCGGGAAATTGGGCAAAA 50.0 45.0 58.0 58.0 1276-1277 bp 1.0 79.8
ndhH-ycf1_p3 TGCATTGATGGATGGTGAGG TACCGGGAAATTGGGCAAAA 50.0 45.0 57.9 58.0 959-960 bp 1.0 79.7
ndhH-ycf1_p4 CGGTAGCTACCCCTCCAATA CGTCTGTCTGTAAAAGGCGA 55.0 50.0 58.0 57.9 1640-1641 bp 1.0 79.6
ndhH-ycf1_p5 CGGTAGCTACCCCTCCAATA TCGTCTGTCTGTAAAAGGCG 55.0 50.0 58.0 57.9 1641-1642 bp 1.0 79.6