Hotspot

psbL-psbF

LSC · 63487-63513 · 27 bp

Back to Achyranthes

Pi 0.0247

MarkerSeek score 55.70

Primer available yes

Label psbL-psbF

Feature Metadata

label_name: psbL-psbF
feature_type: igs
region: LSC
length_bp: 27
variable_sites: 1
pi: 0.0247
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbL-psbF_p1	TCGTTCGGGTTTGATTGTGT	TGGGGTCAATATCCGCAATG	45.0	50.0	58.0	58.0	88 bp	1.0	80.4
psbL-psbF_p2	TTCGGGTTTGATTGTGTCGT	TGGGGTCAATATCCGCAATG	45.0	50.0	58.0	58.0	85 bp	1.0	80.4
psbL-psbF_p3	CGTTCGGGTTTGATTGTGTC	TGGGGTCAATATCCGCAATG	50.0	50.0	57.7	58.0	87 bp	1.0	78.3
psbL-psbF_p4	GTTCGGGTTTGATTGTGTCG	TGGGGTCAATATCCGCAATG	50.0	50.0	57.7	58.0	86 bp	1.0	78.3
psbL-psbF_p5	TTCGGGTTTGATTGTGTCGT	CTGGCTGTACCTACCGTTTT	45.0	50.0	58.0	57.5	110 bp	1.0	76.6