Hotspot

ndhK-ndhC

LSC · 50180-50229 · 50 bp

Back to Achyranthes

Pi 0.0267

MarkerSeek score 57.00

Primer available yes

Label ndhK-ndhC

Feature Metadata

label_name: ndhK-ndhC
feature_type: igs
region: LSC
length_bp: 50
variable_sites: 2
pi: 0.0267
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK-ndhC_p1	CGGCCATAAACTCGAGAGTC	CATGGCGAAAAGGAGCATTG	55.0	50.0	58.2	58.1	190 bp	1.0	78.9
ndhK-ndhC_p2	CGTAGAGAAGCGGCCATAAA	CATGGCGAAAAGGAGCATTG	50.0	50.0	57.8	58.1	200 bp	1.0	78.4
ndhK-ndhC_p3	AGAGAAGCGGCCATAAACTC	CATGGCGAAAAGGAGCATTG	50.0	50.0	57.7	58.1	197 bp	1.0	77.6
ndhK-ndhC_p4	CGGCCATAAACTCGAGAGTC	GCGAAAAGGAGCATTGGAAT	55.0	45.0	58.2	57.1	186 bp	1.0	72.0
ndhK-ndhC_p5	CGGCCATAAACTCGAGAGTC	TATGCATGGCGAAAAGGAGC	55.0	50.0	58.2	59.0	194 bp	1.0	71.8