Hotspot

trnF-ndhJ

LSC · 48195-48905 · 711 bp

Back to Achyranthes

Pi 0.00188

MarkerSeek score 49.40

Primer available yes

Label trnF-ndhJ

Feature Metadata

label_name: trnF-ndhJ
feature_type: igs
region: LSC
length_bp: 711
variable_sites: 2
pi: 0.00188
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	ACGTAAGGATTATATTGTCCCCA	55.0	39.1	58.0	56.8	833-846 bp	1.0	46.9
trnF-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ACGTAAGGATTATATTGTCCCCA	50.0	39.1	58.1	56.8	797-810 bp	1.0	46.5
trnF-ndhJ_p3	CTGGTAGAGCAGAGGACTGA	ACGTAAGGATTATATTGTCCCCA	55.0	39.1	57.9	56.8	821-834 bp	1.0	45.8
trnF-ndhJ_p4	AAGGGCGAATGAATAGTCGG	ACGTAAGGATTATATTGTCCCCA	50.0	39.1	57.8	56.8	851-864 bp	1.0	45.0
trnF-ndhJ_p5	AAGATGATGCGACAGAAGGG	ACGTAAGGATTATATTGTCCCCA	50.0	39.1	57.7	56.8	866-879 bp	1.0	44.3