Hotspot

petN-psbM

LSC · 27976-29152 · 1177 bp

Back to Achyranthes

Pi 0.0017

MarkerSeek score 59.80

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1177
variable_sites: 3
pi: 0.0017
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ATTGTAAGTCTCGCTTGGGC	AAAACGGTCAGCCAAAATGA	50.0	40.0	58.3	56.4	1285 bp	1.0	65.6
petN-psbM_p2	ATGGGGAAGAAGTGGACTCT	AAAACGGTCAGCCAAAATGA	50.0	40.0	57.6	56.4	1223 bp	1.0	64.9
petN-psbM_p3	ATTGTAAGTCTCGCTTGGGC	AAACGGTCAGCCAAAATGAT	50.0	40.0	58.3	56.2	1284 bp	1.0	63.8
petN-psbM_p4	ATTGTAAGTCTCGCTTGGGC	AACGGTCAGCCAAAATGATT	50.0	40.0	58.3	56.2	1283 bp	1.0	63.8
petN-psbM_p5	ATTGTAAGTCTCGCTTGGGC	AAAAACGGTCAGCCAAAATGA	50.0	38.1	58.3	57.2	1286 bp	1.0	63.6