Hotspot

rbcL

LSC · 55345-56775 · 1431 bp

Back to Achnatherum

Pi 0.00606

MarkerSeek score 48.30

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1431
variable_sites: 13
pi: 0.00606
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	AGCCCGTTGGAAAGTTCAAT	GACTCACGATTACGCCTGTT	45.0	50.0	58.0	58.0	2678-2688 bp	1.0	81.5
rbcL_p2	AATGAGCCCGTTGGAAAGTT	GACTCACGATTACGCCTGTT	45.0	50.0	58.0	58.0	2682-2692 bp	1.0	81.4
rbcL_p3	AATGAGCCCGTTGGAAAGTT	GTTGACTCACGATTACGCCT	45.0	50.0	58.0	58.0	2685-2695 bp	1.0	81.4
rbcL_p4	AGCCCGTTGGAAAGTTCAAT	GTTGACTCACGATTACGCCT	45.0	50.0	58.0	58.0	2681-2691 bp	1.0	81.4
rbcL_p5	AGCCCGTTGGAAAGTTCAAT	TTGACTCACGATTACGCCTG	45.0	50.0	58.0	58.0	2680-2690 bp	1.0	81.4