Hotspot

ndhF

SSC · 103336-105555 · 2220 bp

Back to Achnatherum

Pi 0.00676

MarkerSeek score 47.50

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2220
variable_sites: 22
pi: 0.00676
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AACACCCATCAATGCATGGT	AGGTATATGGTACGGAGGCC	45.0	55.0	58.1	58.1	2956-2987 bp	0.666667	67.6
ndhF_p2	AACACCCATCAATGCATGGT	GGTATATGGTACGGAGGCCT	45.0	55.0	58.1	58.1	2955-2986 bp	0.666667	67.6
ndhF_p3	ACCCATCAATGCATGGTGTT	AGGTATATGGTACGGAGGCC	45.0	55.0	58.1	58.1	2953-2984 bp	0.666667	67.6
ndhF_p4	ACCCATCAATGCATGGTGTT	GGTATATGGTACGGAGGCCT	45.0	55.0	58.1	58.1	2952-2983 bp	0.666667	67.6
ndhF_p5	GCATGGTGTTCTTCGACTGA	GGTATATGGTACGGAGGCCT	50.0	55.0	58.2	58.1	2942-2973 bp	0.666667	66.4