Hotspot

ccsA

SSC · 107516-108484 · 969 bp

Back to Achnatherum

Pi 0.00757

MarkerSeek score 56.70

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 969
variable_sites: 11
pi: 0.00757
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	AAGCATCTCGGTTCGAATCC	GCGTTCAAGGGGTTCTCAAA	50.0	50.0	58.1	58.7	1148-1149 bp	1.0	74.8
ccsA_p2	AAGCATCTCGGTTCGAATCC	TTTGAAAAGGCGTTCAAGGG	50.0	45.0	58.1	57.1	1157-1158 bp	1.0	73.2
ccsA_p3	AAGCATCTCGGTTCGAATCC	AAAAGGCGTTCAAGGGGTTC	50.0	50.0	58.1	59.0	1153-1154 bp	1.0	72.6
ccsA_p4	AAGCATCTCGGTTCGAATCC	GAAAAGGCGTTCAAGGGGTT	50.0	50.0	58.1	59.0	1154-1155 bp	1.0	72.6
ccsA_p5	AAGCATCTCGGTTCGAATCC	TTGAAAAGGCGTTCAAGGGG	50.0	50.0	58.1	59.0	1156-1157 bp	1.0	72.6