Hotspot

atpF

LSC · 33592-34973 · 1382 bp

Back to Achnatherum

Pi 0.00289

MarkerSeek score 60.30

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1382
variable_sites: 6
pi: 0.00289
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	GCGAGCGAAGTAAGTGATCT	TCGAAGGGTTGCCATTAGTG	50.0	50.0	57.8	57.9	1553-1573 bp	1.0	77.9
atpF_p2	GCGAGCGAAGTAAGTGATCT	CGAAGGGTTGCCATTAGTGT	50.0	50.0	57.8	58.2	1552-1572 bp	1.0	77.4
atpF_p3	GCGAGCGAAGTAAGTGATCT	GACTCGAAGGGTTGCCATTA	50.0	50.0	57.8	57.6	1556-1576 bp	1.0	75.6
atpF_p4	GCGAGCGAAGTAAGTGATCT	ACTCGAAGGGTTGCCATTAG	50.0	50.0	57.8	57.6	1555-1575 bp	1.0	75.6
atpF_p5	GCGAGCGAAGTAAGTGATCT	CTCGAAGGGTTGCCATTAGT	50.0	50.0	57.8	57.6	1554-1574 bp	1.0	75.6